Project information
Study of molecular mechanisms causing v-Myb suppression using proteomics-based approach
- Project Identification
- GA301/03/1055
- Project Period
- 1/2003 - 1/2005
- Investor / Pogramme / Project type
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Czech Science Foundation
- Standard Projects
- MU Faculty or unit
- Faculty of Science
- Keywords
- Myb, proteomics, differentiation, tumor suppression
The v-myb oncogene of avian myeloblastosis virus causes acute myeloblastic leukemia in vivo and transforms myelomonocytic cells in vitro. It codes for a v-Myb transcription factor that changes expression pattern of its target genes and/or interacts with other proteins, thus causing transformation of the cell. The model line of v-Myb-transformed monoblasts BM2 can undergo terminal differentiation, growth arrest and/or apoptosis in response to extracellular stimuli, such as phorbol esters, trichostatin A and retinoic acid. Sensitivity of BM2 cells to these agents can be significantly affected by ectopic expression of certain genes (RAR, RXR, c-myb, v-jun, c-jun, v-fos, c-fos, CBP, p300, p53, bcl-2). These effects result from specific interaction of the v-Myb protein and/or its target gene products with cellular signaling pathways directed by these inducers. The aim of this project is to investigate the network of intracellular pathways that possess decisive role in leukemogenesis induced by v-Myb oncoprotein. It takes advantage of avilability of the set of BM2 derivatives expressing single transgenes created in our laboratory and proposes to use them in exploring molecular mechansims responsible for v-myb suppression in BM2 cells by proteomics approach. Comparison of two-dimensional electrophoretograms of proteins purified from BM2 cells induced to differentiate and from untreated controls would suggested the differentially expressed proteins to be identified by mass spectrometry. Understanding of cellular molecular pathways causing suppression of the oncoprotein has important implication for selection of targets for anti-cancer therapies.
Publications
Total number of publications: 23
2005
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Regulace a význam exprese vimentinu při diferenciaci monoblastů tranformovaných onkogenem v-myb
Sborník příspěvků IX. pracovního setkání biochemiků a molekulárních biologů, year: 2005
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THE ROLE OF VIMENTIN EXPRESSION IN MONOCYTE/MACROPHAGE DIFFERENTIATION
XIII. Cytoskeletální Klub, year: 2005
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Versatility of proteome during terminal differentiation of v-myb-transformed monoblasts
Sborník příspěvků IX. pracovního setkání biochemiků a molekulárních biologů, year: 2005
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Versatility of proteome during terminal differentiation of v-myb-transformed monoblasts
Študentská vedecká konferencia, year: 2005
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Využití průtokové cytometrie pro sledování proliferace a diferenciace monoblastů transformovaných onkogenem v-myb
Analytická cytometrie III, year: 2005
2004
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Autofagie je preferovaným typem programované buněčné smrti u monoblastů BM2 stimulovaných kamptotecinem
Česko-slovenská studentská vědecká konference, year: 2004
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Camptothecin induces autophagy of v-myb-transformed monoblasts
Differentiation, year: 2004, volume: 72, edition: 6
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Optimalizace přípravy vzorku pro dvourozměrnou elektroforézu
Chemicke listy, year: 2004, volume: 98, edition: 12
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Příprava vzorku pro analýzu proteomu leukemických buněk BM2 dvourozměrnou elektroforézou
1. česká proteomická konference, year: 2004
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ZVÝŠENÁ EXPRESE VIMENTINU JAKO MARKER DIFERENCIACE MONOBLASTŮ BM2
VIII. Pracovní setkání biochemiků a molekulárních biologů. Sborník abstraktů., year: 2004