Project information
Functional and molecular characteristics of cancer and normal stem cells - identification of targets for novel therapeutics and therapeutic strategies
- Project Identification
- MSM0021622430
- Project Period
- 1/2007 - 12/2013
- Investor / Pogramme / Project type
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Ministry of Education, Youth and Sports of the CR
- Research Intents
- MU Faculty or unit
- Faculty of Medicine
- Other MU Faculty/Unit
- Faculty of Science
- Other MU Faculty/Unit
- Faculty of Informatics
- Other MU Faculty/Unit
- Central European Institute of Technology
- Keywords
- embryonic stem cells
The goal of this project will be a) to profit from availability of unique cellular resources - mouse and human embryonic stem cells and normal and malignant hematopoietic stem/progenitor cells, b) to effectively employ a wide variety of current and complementary methodologies available to the consortium, and c) to exploit a proven/certified expertise in working with various living systems that span from in vitro cultured cells, through animal models to oncohematological patients. All this together will represent a powerful experimental platform aimed at addressing a nature of molecules and molecular mechanisms underlaying phenomena that are common to both normal and cancer stem cells, mainly their ability to self-renew and to enter differentiation pathways. Integral part of this general goal is to implement multifaceted analytical approach that will cover studies of gene expression at both mRNA and protein levels, evaluating function of extra and intracellular signaling pathways, and analyzing biological properties of cells in vitro and in vivo. Expected outcome will be not only understanding but also a definition of molecules and/or molecular pathways to be tested as therapeutic targets, and cellular models that will make such testing feasible.
Publications
Total number of publications: 357
2010
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Breast cancer-specific mutations in CK1epsilon inhibit Wnt/beta-catenin and activate the Wnt/Rac1/JNK and NFAT pathways to decrease cell adhesion and promote cell migration.
Breast Cancer Research, year: 2010, volume: 2010, edition: 12/3, DOI
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CLL-specific resequencing microarray as a tool for CLL research and diagnostics
Year: 2010, type: Conference abstract
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Clonal evolution of malignant populations in potentially biclonal chronic lymphocytic leukemia patients.
Year: 2010, type: Conference abstract
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Co víme o incidenci chronické lymfocytární leukemie (CLL), a kde a jak jsou nemocní s CLL léčeni?
Transfuze a Hematologie dnes, year: 2010, volume: 16, edition: 1
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Custom microarray for non-hodgkin lymphoma classification
Year: 2010, type: Conference abstract
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Dasatinib Promotes BIM Dependent Apoptosis Via MEK/ERK without Need for Permanent BCR-ABL Inhibition
Year: 2010, type: Conference abstract
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Detail characterization of 22q11 deletion and miR-650 role in CLL patients
Year: 2010, type: Conference abstract
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Detection of an aberrant form of E13A2 BCR-ABL transcript causing failure of molecular diagnostics in patient with chronic myeloid leukemia (CML)
Year: 2010, type: Conference abstract
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Determination of pCrkL level in chronic myeloid leukaemia cells using flow cytometry
Advances in Molecular and Cancer Biology, 2010, year: 2010
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Difficulties in using 1,3-ß-D glucan as the screening test for the early diagnosis of invasive fungal diseases in patients with hematological malignancies - high frequency of false positive results and their analysis.
Journal of Medical Microbiology, year: 2010, volume: 59, edition: 9, DOI