Project information
Dispersion polymerization of hydrofilic monomers for the preparation of magnetic carriers used in biotechnological application
- Project Identification
- GA203/00/1339
- Project Period
- 1/2000 - 1/2002
- Investor / Pogramme / Project type
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Czech Science Foundation
- Standard Projects
- MU Faculty or unit
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Faculty of Science
- doc. Ing. Bohuslav Rittich, CSc.
- Cooperating Organization
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Institute of Macromolecular Chemistry of the ASCR, v. v. i.
- Responsible person Ing. Daniel Horák, CSc.
Project is aimed at the design of new hydrophilic magnetic carriers of spherical shape and uniform size in range of micrometers, which can be used in biotechnological applications. The carriers will be prepared by dispersion polymerization in the presenc e of various magnetic iron oxides. The effect of type and amount of iron oxide added, solvent mixture, monomer concentration, type and concentration of stabilizer and initiator and temperature on the properties of the resulting particles will be determin ed and the polymerization mechanism elucidated. Various affinity ligands, such as enzymes, antibodies, or reactive dyes will be bound to modified magnetic particles. For example, RNase, proteinase, lysozyme, or antibodies against some pathogenic bacteria will be immobilized on the magnetic particles. Activity of immobilized proteins will be tested in the preparation of crude cell lysate, or by means of immunomagnetic separation. Particles with immobilized bacteria will be used in polymerase chain reacti
Publications
Total number of publications: 18
2001
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Properties of nucleases immobilized on hydrophilic polymer particles
Separation in the BioSciences SBS 2001, year: 2001
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Properties of RNase A immobilized on magnetic poly(2-hydroxyethyl methacrylate) microspheres
Biotechnology Progress, year: 2001, volume: 17, edition: 3
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The influence of metal ions on the activity of immobilized nucleases
Life Sciences 2001, year: 2001
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The limitation of PCR in identification of Salmonella cells in foods
Life Sciences 2001, year: 2001
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Use of enzymes and proteins immobilized on magnetic polymer particles in bacterial molecular diagnostics
Separation in the BioSciences SBS2001, year: 2001
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Vlastnosti DNázy I imobilizované na magnetických nosičích
V. Pracovní setkání biochemiků a molekulárních biologů, year: 2001
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Vnitrobuněčné inhibitory PCR v baktériích Salmonella typhimurium
V. Pracovní setkání biochemiků a molekulárních biologů, year: 2001
2000
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DNaseI immobilized on magnetic hydrogel particles in DNA analysis
20th International Symposium on the Separation and Analysis of Proteins, Peptides and Polynucleotides, year: 2000