Project information
Application of computer image analysis in optical microscopy
- Project Identification
- MSM 143300002
- Project Period
- 1/1999 - 12/2004
- Investor / Pogramme / Project type
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Ministry of Education, Youth and Sports of the CR
- Research Intents
- MU Faculty or unit
- Faculty of Informatics
- Keywords
- image analysis; image processing; segmentation; preprocessing; deconvolution; 3D analysis; 3D reconstruction; mathematical morphology; parallel computing; parallel algorithms; optical microscopy; confocal microscopy; automation; automated microscopy
Aim: The aim of the project is the research in the field of computer analysis of microscopic images, in the field of automation of the acquisition process and on-line image analysis and application of gained knowledge in praxis. The proposal assumes both continuation of running research of cell nucleus structure with clinical applications in diagnostics and prevention of leukemias and other cancer diseases as well as extension of the research into other application areas of computer analysis of microscopic images, biomedical as well as industrial. Content: Especially the algorithms of image preprocessing and segmentation will be studied with emphasis on deconvolution of 3D images, mathematical morphology operations and 3D image reconstruction from a series of 2D image slices. An intensive research will be led also in the area of parallel image analysis algorithms and parallelization of selected algorithms on multi-processor workstations using the equipment of Supercomputing centre MU.
Results
The aim of the project is the research in the field of computer analysis of microscopic images, in the field of automation of the acquisition process and on-line image analysis and application of gained knowledge in praxis.
Publications
Total number of publications: 88
2002
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Chromatin modifications determined in retinoblastoma
Year: 2002, type: Conference abstract
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Nuclear structure and gene activity in human differentiated cells
Journal of Structural Biology, year: 2002, volume: 139, edition: 2
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Nuclear topography and gene activity
Biophysics of the genome and its interactions, year: 2002
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Optimisation of FISH Methodology for the Study of 3D Structure of Cell Nuclei Using Micro Axial Tomography
Biophysics of the Genome and Its Interactions, year: 2002
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Spatial distribution of centromeric and subtelomeric DNA sequences in interphase nuclei of human Go and stimulated lymphocytes
Biophysics of the genome and its interactions, year: 2002
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Structure of the human genome: Experimental results and models
Biophysics of the genome and its interactions, year: 2002
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The 3D structure of human chromosomes in cell nuclei
Chromosome Research, year: 2002, volume: 10, edition: 7
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The power of computerized light microscopy
Imaging & Microscopy, year: 2002, volume: 4, edition: 2
2001
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Combined confocal and wide-field high-resolution cytometry of FISH-stained cells
Cytometry, year: 2001, volume: 45, edition: 1
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Cytogenetics of Retinoblastoma
Year: 2001, type: Conference abstract