Project information
Application of computer image analysis in optical microscopy
- Project Identification
- MSM 143300002
- Project Period
- 1/1999 - 12/2004
- Investor / Pogramme / Project type
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Ministry of Education, Youth and Sports of the CR
- Research Intents
- MU Faculty or unit
- Faculty of Informatics
- Keywords
- image analysis; image processing; segmentation; preprocessing; deconvolution; 3D analysis; 3D reconstruction; mathematical morphology; parallel computing; parallel algorithms; optical microscopy; confocal microscopy; automation; automated microscopy
Aim: The aim of the project is the research in the field of computer analysis of microscopic images, in the field of automation of the acquisition process and on-line image analysis and application of gained knowledge in praxis. The proposal assumes both continuation of running research of cell nucleus structure with clinical applications in diagnostics and prevention of leukemias and other cancer diseases as well as extension of the research into other application areas of computer analysis of microscopic images, biomedical as well as industrial. Content: Especially the algorithms of image preprocessing and segmentation will be studied with emphasis on deconvolution of 3D images, mathematical morphology operations and 3D image reconstruction from a series of 2D image slices. An intensive research will be led also in the area of parallel image analysis algorithms and parallelization of selected algorithms on multi-processor workstations using the equipment of Supercomputing centre MU.
Results
The aim of the project is the research in the field of computer analysis of microscopic images, in the field of automation of the acquisition process and on-line image analysis and application of gained knowledge in praxis.
Publications
Total number of publications: 88
2000
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An efficient algorithm for measurement and correction of chromatic aberrations in fluorescence microscopy
Journal of Microscopy, year: 2000, volume: 200, edition: 3
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Chromosomes participating in translocations typical of malignant haemoblastoses are also involved in frequent interchanges induced by densely ionising radiation
Year: 2000, type: Conference abstract
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Influence of cell fixation on chromatin topography
Analytical Biochemistry, year: 2000, volume: 282, edition: 1
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Nuclear topography of the c-myc gene in human leukemic cells
Gene, year: 2000, volume: 244, edition: 1
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Spatial arrangement of genes, centromeres and chromosomes in human blood cell nuclei and its changes during the cell cycle, differentiation and after irradiation
Chromosome Research, year: 2000, volume: 8, edition: 6
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The influence of the cell cycle, differentiation and irradiation on the nuclear location of the ABL, BCR and C-MYC genes in human leukemic cells
Leukemia Research, year: 2000, volume: 24, edition: 3
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Topography of genetic loci in tissue samples: towards new diagnostic tool using interphase FISH and high-resolution image analysis techniques
Analytical Cellular Pathology, year: 2000, volume: 20, edition: 4
1999
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Aberrations induced in chromosome 1, 2 and Y of human lymphocytes by three types of radiation of different LET value as detected by FISH
Fundamentals for the assesment of risks from environmental radiation, year: 1999
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Advances in computer analysis of radiation-induced changes in human genome
Fundamentals for the assesment of risks from environmental radiation, year: 1999
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High-resolution cytometry of FISH dots in interphase cell nuclei
Cytometry, year: 1999, volume: 36, edition: 4